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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore may possibly add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two additional splice variants ended up claimed but specific nucleotide sequence details isn't nevertheless accessible.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.
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This guidebook outlines more doable optimization of cellular and immunoassay parameters to ensure the very best effects are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...
Antibodies bound to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is specific.
Target Data Cyclin E belongs to the hugely conserved cyclin relatives, whose members are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation styles which contribute to the temporal coordination of every mitotic event. Cyclin E sorts a complex with and features to be a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S transition. This protein accumulates on the G1-S section boundary and is also degraded as cells progress by means of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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